The precursor ion intensity threshold was set to above 1000 counts.
The precursor mass tolerance threshold was 10 ppm and the maximum fragment mass error 0.8 Da.
Furthermore, if the volume percentage (vol.%) of OLA in the precursors exceeded a threshold of 37.5%, the resulted CdSe NCs demonstrated long-term fixing of size distribution up to 300 s.
Cells chosen to be precursors sustain high threshold levels of expression and signal to the cells surrounding them causing them to gradually lose proneural gene expression [1].
Data-dependent MS/MS data were acquired using the following additional parameters: number of precursors, 3; absolute threshold, 2000; summation factor, 1.0×.
Precursors above the threshold of 5000 counts were isolated within a 2 Th window and fragmented by CID in the LTQ Velos using normalized collision energy of 35 and an activation time of 10 ms.
The threshold for precursor ion selection was 500 and mass window for precursor ion selection was 2.0 Da.
MS/MS data were processed with in-house Bioworks (version 3.2, Thermo Electron Corporation, San Jose, CA) as described below: MS/MS spectra were extracted from the raw files by using SEQUEST with the following parameters; MW range: 250 4,000; threshold, 1,000; precursor mass tolerance: 2; group scan tolerance, 2; minimum group count, 1; precursor charge state, auto; MSn level, auto.
It was found that average turbulent velocity fluctuations greater than the laminar flame speed by an order of magnitude were required in order to maintain wave velocities above the Chapman Jouguet (CJ -deflagration veloCJ -deflagrationa precursor requirement for detonation re-initiation to occur.
MS/MS spectra were extracted from the raw data files by TurboSEQUEST™ (licensed to Bioworks 3.3) with the following parameters: molecular weight (MW) range, 400 4000; threshold, 0; precursor mass tolerance, 1.4; fragment ion tolerance, 0.5.
