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The resultant measure, the mTCNS, maintains acceptable correlation with the precursor score, the TCNS.
Interestingly, several of the ESTs encoded proteases with possible signal/secretion peptides, including subtilisin-like serine protease (score 0.882), cathepsin-like cysteine protease (score 0.861), calcium-dependent cysteine protease (score 0.782) and metacaspase 1 precursor (score 0.923).
In particular, the sensory test domain of the mTCNS shares moderate correlation with the sensory nerve conduction study parameters and is consistent with earlier reports of construct validity for the precursor score, the TCNS [ 10].
This study demonstrates that the modification of a well-validated score, designed to capture the manifestations of the early processes of DSP better than the precursor score, produces a measure with similar internal consistency, inter-rater reliability and intra-rater reliability.
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(Most actresses who earn that many nominations and key precursors easily score an oscar nomination. But Diaz was snubbed once again.
Note that mir-433-5p mir-433-5p mir-433-5pp" set wasreas mir-433-3placedn the "not enrinhed" sethe topsetthe same precursor whereasred mir-433-3p mir-433-3p mir-433-3p two disferent mature sequences).
All precursors with scores above the threshold were, thus, regarded as putative miRNAs.
The algorithm, as the original percolator algorithm [104], is based on a semi-supervised SVM approach, and is able to discriminate between correct and incorrect identifications by assigning weights to a number of features such as: Mascot score, precursor mass error, fragment mass error, number of variable modifications used in the search, etc.
The lower scoring precursor in each of these cases was excluded from further analysis.
Peptide mapping of the tryptic digest identified it as complement component 3 precursor (estimated Z-score 1.57, 4% sequence coverage).
As mentioned above 354 predicted precursors possessed a score above the cut-off -3, of which an estimated 140 were false positives.
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