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In the resulting subset, predictions were ranked according to precursor positions and sequence similarity, and to target identity.
Potential novel miRNAs were discarded from the final collection when they were reported as deriving from heterogeneous precursor positions or there was no clear dominance of their specific sequence from one arm of the proposed hairpin structure.
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One example alignment and predicted precursor position is shown in Figure 2A.
The overall predictions are ranked in 16 precursor position groups.
The genome locations of novel miRNA precursors were annotated by comparing the precursor position with the latest maize genome annotation (5b.60, www.maizesequence.org).org
Predictions were grouped according to target identity, precursor position in the genome, and precursor sequence similarity, and results were stored in the database.
For example, hsa-mir-376a-1_49_A_g is used to mean an A-to-I editing detected at the position 49 of the hsa-mir-376a-1 hsa-mir-376a-1 hsa-mir-376a-1 hsa-mir-376a-1uence is "A" and the edited reads have "g" at this site.
In this simple and generally elegant model, the anticodon precursor is positioned in the same place as in our model adjacently to the 5'DCCA3'.
Finding such cost-effective solutions is an important precursor to positioning franchise networks for longer-term sustainability.
An overview of precursors and miRNA expressed in the goat mammary gland at peak lactation was thus produced and each precursor was positioned on goat chromosomes.
After scanning the entire precursor, each position in the vector is normalized by dividing its counts by the length of the sequence.
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CEO of Professional Science Editing for Scientists @ prosciediting.com