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We then measured the expression of nestin, a neuronal precursor marker in the different cultures.
The neural and endothelial precursor marker, nestin, was weakly expressed in the original (primary) tumor (0 1+).
Sorting of cells expressing the neural precursor marker Sox1 before transplantation has been shown to further reduce the risk of teratoma formation [10], [11].
These neurospheres were mainly constituted by neuronal precursor cells as shown by immunostainning for neural precursor marker Musashi-1 and neuronal immature marker TUJ.
Double immunostaining of Notch1 with commissural precursor marker Pax7 [24], [24] or commissural neuron markers TAG1 and DCC [25] was performed on the hindbrain sections from HH20 chick embryos.
We have preliminary results showing that another neural precursor marker, the translocator protein (TSPO) is also expressed in both type of cultured melanocytes at the mRNA level (data not shown).
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By looking at all the blood data, it can identify certain precursor markers that suggest an infection is rising.
hESC- as well as hiPSC-derived neural stem/progenitor cell (NSC/NPC) colonies formed rosettes and expressed neural precursor markers Nestin and Sox2.
Neural stem/progenitor cell (NSC/NPC) colonies, showing rosette structures, are positive for neural precursor markers Sox2 and Nestin, whereas neurons are positive for Tuj1 (Bar: 100 or 50 μm).
NS cells also express the neural precursor markers Sox2, Sox3, and Emx2, and the bHLH transcription factors Olig2 and Mash1.
Other studies revealed that neuroblastoma cells express neural precursor markers including CD34, ABCG2, and nestin [7], [16], [18], [30].
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