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It is possible that WT1 serves as a precursor for each of the smaller transcripts, but this cannot be directly inferred.
Existence of a solution implies that the stoichiometric array describes at least one uninterrupted pathway that leads to the transformation of externally supplied substrates into biomass precursor for each of the precursors.
To determine if these miRNAs were also present in other insects, we used BLAST searches with the mature microRNA sequence to hymenopteran genomes, and then examined the presence of a stem-loop structure in the predicted precursor for each significant hit to determine if they are likely to represent orthologous miRNAs.
Currently, all of the drugs in clinical use target the mature RT p66/p51 heterodimer, however, a single p66 peptide chain functions as the precursor for each subunit of the RT heterodimer, requiring a complex maturation process that includes subunit-selective elimination of a single ribonuclease H (RH) domain.
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For more details, see Table 1 to clarify the concentrations and precursors for each sample.
If all four potential precursors for each tag agreed with this criterion, we chose the sequence with the lowest free energy of folding.
Where appropriate both doubly and triply charged precursors for each peptide were tested even if an MS/MS spectrum for just one precursor was available in the library.
An important contribution of this work is the identification of precursors for each of the rice predicted miRNAs coupled to a fine analysis on their genomic organisation.
In three cases, namely cca-miR160, cca-miR169a, and cca-miR395b, two distinct sequences corresponding to putative precursors for each miRNA were found, therefore, following instructions in miRBase, we considered them as distinct miRNAs: cca-miRNA160-1 and 160-2; cca-miRNA169a-1 and 169a-2; cca-miR395b-1 and cca-miR395b-2 cca-miR395b-2 cca-miR395b-2
In this study, estimates of various enzyme activities in purine pathways were calculated using the product to precursor ratios for each enzyme.
T cell precursor frequencies for each peptide epitope were based on the total number of cells and the number of spot forming cells (SFC) per well (average of 3 wells).
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