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For the DBP precursor analysis, the pH value of water samples was kept at 7.0 ± 0.2 by first adding H2SO4 or NaOH (Merck, Germany) solution, and followed by adding adequate amount of phosphate buffer solution (0.8 M, Merck, Germany).

In addition to the precursor analysis, the length and the abundance of new mature miRNA was also examined.

Based on product ion and precursor ion analysis of head groups and fatty acyls, a comprehensive list of MRM transitions was then generated to follow fatty acyl compositions of these lipids (parent → fatty acyl fragment transitions) (Figure 2) [23].

Additional Methods, genome mining of lanthipeptide gene clusters, annotations of lanthipeptide MS/MS spectra, MS/MS and precursor peptide analysis of informatipeptin, spectral networks of MS/MS data sets for lanthipeptide characterization, informatipeptin gene cluster analysis.

The limit of sensitivity of the standard CTL-precursor analysis is one out of 26 063 (Taswell, 1981), whereas the limit of sensitivity of the new method used in this study is one out of 100 000 (Hida et al, 2002).

It means that these different anomalies dates between LAI precursors can be a hint of truthfulness of multi-precursors analysis.

For computing frequency of stable and unstable real precursors, analysis was done on a sample of 500 plant miRNA precursors of varying lengths.

The cost of the standard CTL-precursor analysis is more than 10 times that of the method used in this study (approximately $500 vs $30 ,per peptide, respectively), and the standard CTL-precursor analysis requires large number of feeder cells so that the influence of allogeneic feeder cells may not be entirely excluded.

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