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Pre-amplification of template cDNA using a preliminary PCR step of 14 cycles improves both the accuracy and precision of the transcript quantification using the dynamic arrays (Table 1).
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We present two aspects of the results: firstly the effect that RABT has on the sensitivity and precision of the final transcript set, and secondly the correlation between true and estimated expression of the TP transcripts and the overall FP ¯ / TP ¯ ratio.
We also observe saturation for the precision of the estimated transcript sets.
Therefore, although our empirical method loses sensitivity by taking the proposed statistical tests, the precision of transcript border identification is highly increased.
At copy numbers mimicking medium to high abundance transcripts, the precision of the BioMark arrays is in a similar range to the minimum variation of ~ 0.1 units observed for another nanofluidic PCR array, the OpenArray format [ 14].
The data indicate the quantitative precision of the sequence-based approach for identification of differentially expressed transcripts.
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