A system of three precision motorized stages allows an almost complete scan of the detector.
According to the sum gray value of the image which subtracted each other at the balancing position and bending position to control a high precision motorized rotation stage revolve make the sum gray value remained in the balancing position always, then the motorized rotation stage revolving angle is just the cantilever bend angle.
In brief, the AR-PAM system consists of a tunable pulsed optical parametric oscillator (OPO) laser (Vibrant 355 II HE, Opotek, Carlsbad, USA), a focused ultrasound transducer (V315-SU, Olympus IMS, Waltham, USA; central frequency: 10 MHz; fractional bandwidth: 6 MHz; N.A.: 0.4), and a precision motorized 3D scanning stage (PSA2000-11, Zolix, Beijing, China).
Representative large scale images were acquired using a customized video-rate multiphoton microscope [59] equipped with a custom automated high precision motorized stage (Applied Precision LLC, Issaquah, WA), which allows for the automatic acquisition of ultra-large field image mosaics in 2 and 3 dimensions [57], [58].
The instrument included a precision motorized X– Y sample stage for automated mapping at spatial resolution down to less than 1 μm and extensive software support (LabSpec 4.18) for data processing.
Cells were imaged using a spinning disk confocal microscope (Zeiss, Thornwood, NY, USA), equipped with a CSU-X1A 5000 spinning disk unit (Yokogowa Electric Corporation, Tokyo, Japan), multilaser module with wavelengths of 458, 488, 514 and 561 nm, and an Axio Observer Z1 motorized inverted microscope equipped with a precision motorized XY stage (Carl Zeiss MicroImaging, Thornwood, NY, USA).
This technique offers several advantages over previously applied methods: firstly, by using an experimental set-up including an automated focus-hold system and a high-precision motorized scanning table, hundreds of cells can be analysed within a few minutes.
We captured single images of each well using a DFC340 FX camera and a Z16 dissecting microscope (Leica Microsystems, Inc., Buffalo Grove, IL), a Bio-precision motorized stage with adaptors for the 96-well plates and stage fittings (Ludl, Inc., Hawthorne, NY), and Surveyor software from Media Cybernetics, Inc. (Warrendale, PA).
The incubator system was equipped with a Nikon inverted microscope (TE2000-E, Nikon, Tokyo), which incorporates a high precision motorized-focus and vibration-free optical path changeover mechanism that facilitates image capture in 3D.
The station includes∶ an Olympus IX-70 inverted fluorescence microscope with custom optical filters, and precision XYZ motorized stage, O2 Silicon Graphics computer work station with image collection and deconvolution software.
Thus, this design has wide applications in various fields, especially for monitoring the displacement and vibration of precision-motorized stages.
