Based on the precision in read mapping determined a posteriori, reads within a ± 2-bp window were merged into the nucleotide position most commonly designated as the Ves insertion site.
Overall, taxator-tk showed high precision in simulated read assignment: the macro-precision for all short read lengths remained above 74% and was 82 99% for the genus to kingdom ranks, about 10% lower on average than for the 16S data.
The larger random error is likely due to the lower precision of the aneroid barometer and to possible human error in reading the result.
From our results it is evident that expression level and sequencing coverage are both important determinants of the precision of the gene expression measurement since the variation in read counts between libraries is reduced as read count increases.
The only species not discovered by MetaCluster 5.0 appears in three clusters and all these three clusters have high precision (>90% reads in these clusters are from the missing species).
Therefore the reported results, especially on precision, must be read with care in light of potential optimization.
On the other hand, some studies suggest that Newbler (the 454 assembler) may outperform SeqMan in terms of precision on short reads produced by 454 sequencing because the SeqMan algorithm has been developed and optimized for relatively longer reads from Sanger sequencing [34].
In order to increase the precision of mapping reads, the reads were initially classified by sample and assembled to generate contigs that could be more easily mapped to the reference M. tuberculosis genomes.
In particular, the HiSeq boasts lower error rates, better precision, and longer reads than the GAIIX [ 7].
Delighted by its descriptive precision, Wardley-Fish latereadsds Theophilus Hopkins's book aloud to his mystified fiancée, Melody Clutterbuck, as they ride in a carriage through Hyde Park.
At the same time, she notes everything with precision: she sees, reads, hears, smells, tastes and touches, and there's an exhilarating mix of sophistication and rawness in the writing.
