Hitherto, single water-in-oil emulsion droplets handled on-chip had been the only well-established format that combined high-precision assays in monodisperse compartments with ultrahigh throughput (>10) multistep processes.
The method has the requisite accuracy, sensitivity and precision to assay PMZ in syrup samples and biological fluids.
The method has the requisite accuracy, sensitivity and precision to assay DXM in pharmaceutical products.
Second, the analytical precision of the assays varied, and this may have influenced our results.
The precision of the assays was determined by sample coefficient of variation (SCV).
Inter-assay precision of ELISPOT assays was validated as described previously [ 14].
The intra-assay precision of these assays range from 4.4 to 7.6% coefficient of variation (average 5.6% coefficient of variation).
The levels of serum b-FGF and VEGF were determined by the ELISA method (Parameter kit, Bender Med Systems, Vienna, Austria): b-FGF (sensitivity 30pg/ml; intra-assay precision 10%, inter-assay precision 10%) and VEGF (sensitivity 14pg/ml; intra-assay precision 6.8%, inter-assay precision 8.3%).
Herein, we describe the implementation of such a COD platform to perform high precision nanoliter assays.
The intra-assay precision for this assay is 3.5%, and the interassay precision is 12%.
