Single-cell analyses of transcript and protein expression profiles — more precisely, single-cell resolution analysis of molecular profiles of cell populations — have now entered the center stage with widespread applications of single-cell qPCR, single-cell RNA-Seq and CyTOF.
However, because one of the unique properties of tightly packed microelectrodes in MEA methods is to investigate spatial heterogeneity of these exocytotic events at the single-cell level, combining other techniques to precisely attach single cells and to culture single cells on the surface of MEAs is of interest.
We would definitely agree with the reviewers that our experimental system does not allow us to make the statement that we are precisely quantifying single molecule force, or more specifically single bond rupture, during the activation of BCRs.
Whereas, in general, multidimensional measures capture concepts more precisely than single indicators, this is not the case for wellbeing as single items are also valid and reliable (George 2010).
Traditional DNN based video prediction algorithms wholly and coarsely forecast the next frame, but the proposed video prediction algorithms severally and precisely anticipate single pixel of future frame in order to achieve high prediction accuracy and low computation cost.
The last ∼500 to 300 years in the 14C calibration curve is problematic with wide fluctuations often rendering large age spans that do not precisely date single events, especially troubling with a culture-historical record of ∼1000 years duration.
This tray enables rapid ex vivo configuration of the fibula segment(s) with the reconstruction bar relative to the native mandibular segments and allows the compiled construct to be transferred to the head and neck for insetting as a precisely configured single unit.
