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The use of microCT to detect lesions in mice offers numerous advantages over traditional techniques. 1) From the perspective of the researcher, hands-on time is possibly reduced; the aortas need not be precisely removed from the animal and only minimal organ trimming is necessary.
In both cases a URA3 gene from which the antisense intron had been precisely removed was detected.
Because of the blunt termini created by the Pwo polymerase, the ligated product excludes the intervening sequence between the divergent primers and, in our case, precisely removed the intron sequence as it would be in the processed mRNA.
The single intron loss found in our study lends support to the 'intron exclusion hypothesis', which suggests that a single intron could be precisely removed by double strand breaks from a multiple-intron gene [ 67].
To determine whether isthmic Atoh1 expression is dependent on signalling from the isthmic organiser, we designed a series of in vitro explant experiments in which different tissues were selectively and precisely removed (Fig. 3A).
Because they are transcribed, if gene function is to be maintained, such introns must be precisely removed from pre-mRNAs (premature messenger RNA) by the spliceosomal machinery after transcription (Berget et al. 1977; Chow et al. 1977; Goldberg et al. 1977).
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In our ongoing breeding program, we are using RICE6K to examine the genetic background of the selected lines and to more precisely remove the negative genetic drags.
This paper proposed a concept design of novel endoscopic machining tool, which aims at quantitatively and precisely removing material from imperfect components in hard-to-reach cavities, such as turbine blades in a jet engine.
Because there is no mechanism available for precisely removing junk DNA, it accumulates by Muller's ratchet (Muller 1964).
We then tested the specific requirement both for H1 and H2 (H2a or H2a and H2d), by precisely removing either helical domain in Col or EBF* (ColΔH1/ColΔH2a and EBF*ΔH1/EBF*ΔH2 proteins, respectively Fig. 3A).
Stable binding of the NTC is required for the formation of a catalytically active spliceosome that is competent to precisely remove introns from precursor mRNA (pre-mRNA) to form mature message (mRNA).
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