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Thus far, there is no animal model to precisely dissect cell type specific IL-1 actions.
The current study was designed to precisely dissect the signaling pathway(s) responsible for the naphthalimide-induced cell cycle arrest in human colon carcinoma HCT116 cells.
The photostability, fast kinetics and sensitivity of this dye are highly advantageous for recording spontaneous action potential firing of multiple neurons, an essential requisite to precisely dissect neuronal networks.
High-throughput genotyping technology and phenotyping platforms have enabled large-scale marker-trait association analysis, such as genome-wide association studies, to precisely dissect the genetic architecture of plant traits.
To precisely dissect the fluorescence events related to complex formation and pinpoint uniquely the contribution of the tryptophan found in IFNγ, we treated LcrV with N-Bromosuccinimide (NBS) [32], in order to chemically modify its Trp 113.
The vector set presented here (See Fig. 1 for a general overview) is a general platform which can be used to precisely dissect cis-regulatory sequences, and to overexpress wild type or tagged proteins in a variety of chordate systems.
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Liver, caecum (full and empty), muscles (vastus lateralis), and adipose tissues (epididymal, subcutaneous and visceral) were precisely dissected and weighed.
However, precisely dissecting the role of these alleles in resistance may also be hindered by the presence of interfering dominant resistances in the genetic background [ 35].
We employed the vervet monkey as a non-human primate model to assure precisely dissected high-quality brain tissues, which are difficult to obtain from human subjects.
Analysis of blood and high-quality brain tissues from precisely dissected regions revealed that levels of almost all these ubiquitous transcripts vary between at least one pair of tested tissues in the current study.
Next, we performed a time-course analysis with CNS tissue that was more precisely dissected into endothelial cells (CD31), astrocytes (GFAP) and "other" parenchymal cells (selected areas devoid of CD31 and GFAP immunostaining) by LCM.
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