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In a statement issued within hours of the piece's publication, the Vatican spokesman, Federico Lombardi, said: "As has happened previously in an analogous situation, it needs to be noted that that which Scalfari attributes to the pope, by putting his words into inverted commas, is the product of his memory as an expert journalist, but not the precise transcription of a recording".
Protein-DNA interactions on DNA cis-regulatory elements and cross-talk between these complexes underlie precise transcription regulation.
It should be noted that the chromatin immunoprecipitation method does not identify the precise transcription factor binding site on the DNA; additional approaches such as EMSA or use of promoter decoys will be required to confirm this point.
Recently, the precise transcription initiation and termination sites have been determined in a genome-wide fashion [ 23, 24].
RNA-seq can also be used to study differential splicing and locate precise transcription start and stop sites, all at single-base resolution (Ozsolak and Milos 2011).
TATA box sequence elements, which were required for critical and precise transcription initiation, were found in the −317 region of the sequence.
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For instance, neuronal activity induces relocalization of gene loci to 'transcription factories', and specific enhancer promoter looping contacts allow for precise transcriptional regulation.
A precise phonetic transcription, in which sounds are described in a great deal of detail, is known as a narrow transcription.
DeepCAGE [ 6, 7] is an improvement over the CAGE (cap-analysis of gene expression) protocol [ 8], which determines precise gene transcription start sites (TSSs) and promoter expression through sequencing of the 5' ends of mRNA transcripts.
By targeting a defined region of cDNAs, the DGETP method can generate wider transcriptome coverage together with a higher number of cDNA tags per gene, leading to more precise gene transcription data.
With a high resolution and sensitivity, the mRNA-Seq can provide detailed information on transcriptional structure of genes such as the precise location of transcription boundaries to a single-base resolution, reveal rare transcripts or variants, and identify splicing isoforms of known genes (Wang et al. 2009a).
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