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Precise transcript distribution in the syncytium is recovered via straightforward spatiotemporal averaging, i.e., accumulation and diffusion of transcripts during nuclear cycles, without regulatory feedback.
A significant deficiency of microarrays is their limited ability to discriminate variation in the precise transcript expressed.
Precise transcript processing is essential for normal plant development, and its relevance to abiotic stress tolerance was exemplified by the identification of the STA1 Arabidopsis mutant, since plants possessing defective sta1 alleles exhibited mis-splicing of COR15 transcripts under cold stress [ 57].
Similarly, each mRNA is assembled into an RNP (mRNP) particle involving a series of complex assembly intermediates, which associate with each species of RNA in a dynamic fashion to allow for precise transcript maturation (Fatica and Tollervey 2002; Hopper and Phizicky 2003; Vinciguerra and Stutz 2004).
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More precise transcripts would be confirmed in coming gene cloning and functional research in bamboo.
Such enhancer sharing can result in similar expression patterns, and may lead to selective advantage by allowing more precise transcriptional control of similar transcripts that act in a combinatorial manner, such as the Hox genes [ 56- 59].
Taken together, transcriptome profiling through RNA-Seq provides an excellent approach for the precise assessment of transcript levels and transcript isoforms in the Drosophila model of infection and immunity.
Compared with long standing methods such as microarray, RNA-Seq gives a far more precise measurement of transcript expression levels and a far more sophisticated characterization of transcript isoforms [ 9, 10].
Characterization of RNA transcripts by length does not have the resolution to identify precise sites of transcript initiation or termination, precise splice sites, or even exon-intron structure, but it does provide an unbiased measurement of transcript length, a quantity that is relatively difficult to obtain through full-length cDNA sequencing alone.
It turns out that all considered methods except MMonly are comparably precise at larger transcript concentrations csp-in > 2 pM, at which the transcripts are safely called present (see previous paragraph).
A more precise measurement of transcript abundance of these genes was performed by qRT-PCR, which revealed that differential expression was significantly greater than had been detected by microarray: the range of fold difference between ALL and AML by gene expression array was 1.0 2.8 and by qRT-PCR 2.3 45.6 (Figure 3B).
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