Exact(10)
To be precise, replacement is global - the tree's reference to the primitive is replaced by this refinement.
Such model aims at accurately incorporating all the main and unique features of the RNC isolator, away from mathematical complications, to provide a convenient and precise replacement of the RNC isolator in further professional studies and research works by both practicing structural designers as well as academic investigator.
Herein we present the generation of a GFP reporter for ADGF-A expression by a precise replacement of the ADGF-A coding sequence with GFP using homologous recombination.
We used homologous recombination to produce a GFP reporter for the analysis of endogenous, in vivo, levels of ADGF-A expression by the precise replacement of the ADGF-A coding sequence with that of GFP (Figure 1).
Further sequencing was performed to confirm the precise replacement (supplementary material Fig. S2).
This method not only allows for the generation of molecularly defined lesions, but also the precise replacement or tagging of genes in their endogenous loci.
Similar(50)
However, two precise replacements of plArB with p[ w +mC GM roX1 MS2-6] were recovered.
Precise allelic replacement mutagenesis of perR in serotype M1 GAS strain AP1 was performed by replacing the DNA- and metal-binding domains of perR (residues 202 to 361) with a kanamycin resistance gene, and complemented with a plasmid containing a functional perR gene and promoter region (Ricci, Janulczyk and Bjorck 2002).
Rights & permissionsfor article Precise gene replacement in rice by RNA transcript-templated homologous recombination.
The precise gene replacement may lead to the addition of an exogenous active gene (knock in) and the specific animal gene inactivation (knock out).
Precise gene replacement was confirmed by sequencing.
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