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EBS patient keratinocytes with precise modifications of the mutant allele are preferentially recovered from targeted cell populations.
In the process of precise modifications of the Drosophila genome through the HDR pathway by co-injection of a circular homologous donor, two ways of holiday-junction resolution lead to two kinds of outcome: "ends-out" and "ends-in" (Fig. 5; supplementary material Fig. S11).
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It was demonstrated that precise modification of the polymeric surface can be achieved with high pixel dwell time at low ion fluence.
Our nanofilter enables the precise modification of the nanoscale pore surfaces of the formed silica NTs with organic moieties, such as trimethylchlorosilane, leading to the efficient filtration of high concentrations of proteins (retentate ≥ 10−4 mol dm−3) without substantial kinetic hindrance.
As improved tools make transgenesis more and more efficient, the stage has been set for precise modification of the zebrafish genome such as are done in other model organisms.
Another important application of CRISPR/Cas is the precise modification of the genome by HDR.
Captive bottlenose dolphins are capable of adjusting their echolocation click amplitude yet have more difficulties in precise modification of the frequency structure of their clicks (Moore and Pawloski 1990).
Additionally, the optimal assignment on molecular graphs has the advantage to investigate and interpret the mappings, allowing precise modifications of internal parameters of the similarity measure for specific targets.
Our experimental observations and first-principles calculations demonstrated the dynamics, stability, and selectivity of such a single-atom chisel, which opens up the possibility of fabricating certain stable molecular devices by precise modification of materials at the atomic scale.
The generation of targeted mutations and the precise modification of an organism's genome sequence are powerful approaches to characterize the function of genes and regulatory elements.
Therefore, technologies that facilitate the precise modification of cellular genomes have diverse and significant implications in many facets of research and are essential for translating the products of the Genomic Revolution into tangible benefits for medicine and biotechnology.
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CEO of Professional Science Editing for Scientists @ prosciediting.com