Sentence examples for precise insertion from inspiring English sources

The sequence surrounding an inserted ERIC remains unchanged, indicating a precise insertion or deletion event via a mechanism distinct from classic transposition mechanisms [ 23, 24].

A clone with the expected band pattern indicating that the MYC gene had correctly inserted into the Nkx3.1 locus was sequenced to ensure that precise insertion had occurred.

We thus sought to investigate the effects of these minor haplotypes (APOA5*2 and APOA5*3) on ApoAV plasma levels through the precise insertion of single-copy APOA5 haplotypes at a targeted location (Hprt) in the mouse genome.

While CRISPR-based gene knock out in mammalian cells has proven to be very efficient, precise insertion of genetic elements via the cellular homology directed repair (HDR) pathway remains a rate-limiting step to seamless genome editing.

The primary method of deployment was by helicopter, with the SASR working closely with No. 9 Squadron RAAF which regularly provided rapid and precise insertion and extraction of patrols into jungle landing zones at tree top height.

The precise insertion of each construct was confirmed by PCR amplification and Southern Blot analysis (Figure S2).

After showing that both TALEN and CRISPR/Cas9 can efficiently mediate precise genomic modifications such as deletions and nucleotides replacement, we set out to explore precise insertions.

The resulting high HDR frequencies without off-target editing provide an important advance for generating scar-less genetic modifications, including epitope-tagged alleles, reporter genes, precise insertions and deletions and point mutations.

Comparing all experiments using the nhr-25 :: 3xFLAG oligos (Table 2, Figure S6C), both knock-in candidates obtained using the purified oligo were precise insertions, whereas two of the four candidates generated with the unpurified oligo contained additional sequence inserted.

The NHEJ pathway generates variable insertions or deletions (indels) at the DSB, while HDR employs homologous donor DNA sequences from sister chromatids, homologous chromosomes or exogenous DNA molecules to produce precise insertions, deletions or base substitutions at a DSB site or between two DSBs.

Thus, we caution that when complete genome data are available for Peramelemorphia or Notoryctemorphia allowing for a more exhaustive marker screen, it could reach other conclusions, as we cannot completely rule out the possibility of exact deletion or precise insertions of elements in our markers (van de Lagemaat et al. 2005).