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The effects of enzyme dosage and temperature on WAS hydrolysis were further discussed based on the analysis of variance (ANOVA).
Optimal enzymatic hydrolysis conditions for rachis and pseudostem were 17.6% of solid loading and 16.0 FPU.glucanucan of enzyme dosage and 15.1% of solid loading and 14.9 FPU.glucanucan of enzyme dosage, respectively.
Experimental design (cross-classification) was used to examine the influence of enzyme dosage and treatment time on drainage.
The optimum values were found to be pH 8.5, enzyme dosage 0.4% on weight of fabric and temperature 55 °C.
The optimal ratio of these synergistic enzymes is very important in improving hydrolysis efficiency and reducing enzyme dosage but has hardly been addressed in literature.
The optimization of enzyme dosage was conducted to improve the enzymatic reaction (Fig. 6).
Cellulase enzyme dosage levels were varied from 5 to 50 FPU.
At higher FPU levels of enzyme dosage, surfactant addition does not have a significant impact.
In addition, the increase in the enzyme dosages led to the improved yields, and the highest yield of up to 85% was achieved with the enzyme dosage of 4 U/mL.
Designing highly efficient cellulase mixtures ("optimized enzyme cocktails") that can yield high hydrolysis rate at minimal enzyme dosage, is one such approach.
The optimal enzyme dosage was regarded as 4 U/mL, where the S/H was around 1.5.
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