Cell based transgenesis allows investigators to make more precise genetic modification by utilizing natural occurring events within the genome such introduction of double stranded DNA breaks at targeted sites.
All these technical traits largely benefit a precise cell-based assay that could be currently impossible to achieve by using a conventional static cell culture model.
Results revealed that even minor environmental pH change could significantly influence the metabolic activity of the cultured primary cancer cells, demonstrating the importance of stable culture condition for a precise cell-based assay.
At present, the molecular nature of such a mechanism in animal cells is completely unknown, and its elucidation at genomic, proteomic and biochemical levels would be facilitated by use of a rapid method of separating large subpopulations of cells based on precise size measurements.
But targeting cancer cells based on the network configuration of an "average" cancer cell, however precise, may not suffice to eradicate all tumor cells because of the dynamic non-genetic heterogeneity of cancer cell populations that makes them moving targets and drives the replenishment of the cancer attractor with surviving, non-responsive cells from neighboring abnormal attractors.
The precise microenvironments required for optimal expansion or differentiation of stem cells are only beginning to emerge now, and the controlled differentiation of embryonic stem cells based on tissue engineering remains a relatively unexplored field.
Chen Z , RenX, Xu X, Zhang X, Hui Y, Liu Z, et al. Genetic engineering of human embryonic stem cells for precise cell fate tracing during human lineage development.
We anticipated that this study could provide novel insight and more precise guidance for cell therapy based on MSCs, e.g. to introduce MSCs sufficiently close to the disease foci.
In the final 77 patients, EPCs were also identified on the basis of cell surface marker expression (CD133, CD34, and vascular endothelial growth factor receptor-2 [VEGFR-2]) and aldehyde dehydrogenase (ALDH) activity.Endothelial progenitor cell enumeration based on fluorescence activated cell sorting was more precise than culture assays.
Fast and precise algorithm based on maximum radial symmetry for single molecule localization.
