Sentence examples for precipitation protocol from inspiring English sources

Following back-extraction with chloroform, RNA was precipitated using a standard ethanol precipitation protocol, and resuspended in RNase-free water.

A summary of the steps involved in SSH and a more detailed figure of the formation of the ESTs from mRNA can be found in Additional file 1 and 2. DNA was precipitated using a standard ethanol precipitation protocol [ 41].

Then, the precipitation protocol should be optimized for each individual enzyme focused on minimizing both the enzymatic activity remaining in the supernatant and the co-precipitated salt.

Purification of DNA was carried out using a modified salt precipitation protocol as described in [52].

Genomic DNA was isolated following a standard salt and ethanol precipitation protocol.

HEK-293T cells were transfected using a calcium phosphate precipitation protocol while Trans-IT Jurkat (Mirus Bio) was used to transfect Jurkat cells following manufacturer's instructions.

Comparing the result of different precipitation protocols on a 1D gel we decided to homogenise the tardigrades directly in ice cold lysis buffer and avoid any precipitation steps.

In order to design a purification protocol, the behavior of STIL under various extraction or precipitation protocols was first evaluated.

For testing, we used a method adapted from a previously reported elution-precipitation protocol [ 24].

The selected salt-precipitation protocol on whole blood was performed according to the 'Comparative and Molecular Pharmacogenomic Lab' in Tufts University, USA [ 15].

In contrast, the salt-precipitation protocol method involves lysis of the cells, and multiple washings steps followed by salt-precipitation of the DNA.