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Although gypsum has low precipitation kinetics in the DS brine, precipitation will be promoted when crystallization seeds are available (Reznik et al., 2009), as expected within the alluvial aquifer, and thus, it is reasonable to assume that gypsum precipitation proceeds before biochemical reaction.
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As the precipitation proceeded, the higher LE was observed which might be attributed to co-precipitation of BSA because of the gelation of chitosan and alginate.
Precipitation proceeded for a minimum of 1 h at 4 °C with gentle agitation, and precipitated material was centrifuged at 14 000 rpm in a JA-17 rotor for 10 min. The resulting pellets were resuspended in DPBS (∼12 mL) before extracting with 1 1 v/v CHCl3/ nBuOH.
Stirring of the reaction vessel must be continuous and of the right intensity to ensure the precipitation process proceeds as intended.
It may due to overall precipitation reaction that proceeds as below (Davis et al. 1997).
They reproduce the following experimental observations: for a composition close to the A2 solubility limit, precipitation and ordering proceed simultaneously and lead to subsequent coarsening of B2 precipitates.
The precipitation of PbSO4 proceeded smoothly in the presence of PbSO4 nuclei.
The precipitation was allowed to proceed at room temperature for 90 min.
After incubation, 1 mL of pre-chilled acetone was added and the precipitation was allowed to proceed overnight.
The two supernatants were combined, 125 ul of 100% TCA was added, and precipitation was allowed to proceed overnight at -20°C.
According to Li et al. [20] for the Y/Tb binary systems, homogeneous nucleation of Tb(OH CO3 occurs in priority and then the precipitation of Y(OH CO3 largely proceeds via heterogeneous nucleation on already-formed Tb(OH CO3 layer.
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