Sentence examples for precipitation buffer from inspiring English sources

Pellets were resuspended in 200 μl diethylpyrocarbonate-treated water and RNA re-precipitated with 200 μl LiCl precipitation buffer (Ambion, TX, USA) overnight at −20 oC.

The procedure that we arrived at consists in simply adding one volume of a calibrated precipitation buffer, followed by centrifugation to pellet precipitates, and exchanging the buffer (Scheme S1).

The following day, the peptide phages were precipitated from the cell supernatant with phage precipitation buffer (20% (w/v) PEG6000 in 2.5 M NaCl) and redissolved in 300 μL 1× PBS.

RNA was precipitated with half volume of isopropanol and half volume of high salt precipitation buffer (0.8 M sodium citrate and 1.2 M sodium chloride).

Total protein was extracted using radioimmune precipitation buffer protein lysis buffer according to protocols (Beyotime, Shanghai, China).

Radioimmune precipitation buffer is more denaturing than NP-40 or Triton X-100 lysis buffer because it contains the ionic detergents SDS and sodium deoxycholate as active constituents.

Cells from bone-marrow, esophagus (by scratching inner layer) and stomach (by scratching inner part) were washed twice with PBS (phosphate buffered saline) and were lysed in radioimmuno-precipitation buffer (0.1% SDS, 2 mM EDTA, 1% NP-40, 1% sodium deoxycholate, 50 mM sodium fluoride and 100 U/ml aprotinin).

was added to the immune-precipitation buffer.

Next day, beads were precipitated by centrifuging at 1200 g for 5 min at 4°C and washed thrice with the co-immuno-precipitation buffer.

Membrane protein (100 μg) extracted from MDA-MB-453 cells was incubated with 300 μl of immuno-precipitation buffer and 3 μg of GIRK1 primary antibody (1 μg/μl) with shaking at 4 degrees for 1 hour.

To study the effect of chaotropic buffers on whole-blood DNA extraction, 20 μl of salt-precipitation buffer B and 100 μl of PBS were added to 100 μl of whole blood (step is adapted from QIAGEN's DNeasy® Blood & Tissue kit protocol).