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Nuclear samples were precipitated via the acetone precipitation [59] method to increase their concentrations so that 75 µg of nuclear protein could be loaded in each well.
The particles precipitated via SAS contained a curcuminoid content 2 and 31 times higher than the extracts obtained by rotary evaporation and the ethanolic extract, respectively, obtained by PLE.
DNA was precipitated via 10 h incubation at −80°C with glycogen (Ambion), 3M Sodium Acetate pH 5.5 (Ambion), and ethanol.
Protein pellets were washed twice with ice-cold acetone, and precipitated via centrifugation, as previously.
Mitochondria were precipitated via centrifugation at 11000 g for 10 min at 4 °C.
Species that were indirectly immune precipitated via membranes were subsequently recovered by mild elution with an NP-40 containing buffer.
Similar(51)
Poly-AFPOs could be recovered simply by pouring the reaction mixture into methanol in the end of the reaction and separating the precipitated polymer via filtration.
Lysates were precipitated overnight via Ni-NTA resin (Novagen, Madison, WI).
The big dye sequencing reaction product was either ethanol precipitated or purified via PCR Min-elute (Qiagen).
The combination of a network observation of auroral displays via ground-based digital cameras and in-situ observations of precipitating particles via satellites can be a powerful tool for studying low-latitude auroras.
The results are consistent with the overriding hypothesis that aeroallergens may precipitate suicide via mediators of allergic inflammation.
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