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These precipitated materials also appeared when a lower NaBH4, up to 25 wt%, was used but with very low quantity.
The presence of precipitated materials in stability samples for these two compounds caused large variations in the measured drug concentrations that made the data analysis difficult.
While all IB protein was modified to some extent, not all precipitated materials in the inclusion body suspension immediately solubilized at this step.
Although a higher generation efficiency was achieved (>99 %) when 30 wt% of NaBH4 was applied using the same conditions of the first case study, an appearance of precipitated materials (crystals of sodium borates) in the collector (1st GLS) was observed.
Further blotting analysis of the gp78 precipitated materials revealed that p97/VCP and polyubiquitinated proteins were coimmunoprecipitated by the 2G5 antibody, but not by the 1F1 antibody (Fig. 7B).
Apparently, the scavenging process with precipitated materials and/or resuspended sediments is less effective for uranium.
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Bacterial cells were enmeshed within the precipitated material.
The precipitated material was recovered by filtration, washed with deionized water and dried at 100 °C overnight.
In five ACM sections, we observed a precipitated material compatible with calcification (Fig. 2c).
Thermodynamic data for Fe3(OH)8 is for freshly precipitated material.[39] Calculated with aid from SUPCRT92 program.[19].[19]
Regardless of product source, removal of cells, cellular debris, aggregates and precipitated material is usually necessary prior to purification.
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