Sentence examples for precipitated and tested from inspiring English sources

When mutant Taspase1 was precipitated and tested for co-bound wildtype Taspase1 (Fig. 4A), we observed mainly inactive Taspase1 (amount of p50) when compared to autoproteolytically cleaved wildtype Taspase1 dimers (amount of p22).

In the nChIP experiments, a standard curve was generated to calculate percent of precipitated DNA and test the efficiency of each primer set covering the VEGF-A locus.

To test for Red1-Rec8 interaction more directly, we precipitated V5-Red1 from sonicated and nuclease digested extracts using an anti-V5 antibody and tested the precipitate for the presence of Rec8-HA.

Therefore, the precipitated proteins from N2-4 and N3-8 were prepared and tested with some pathogenic bacteria by agar well diffusion.

Student's t-test at P < 0.05 level was used to compare the values between TCA precipitated and control samples.

After immunoprecipitation with anti-GFP antibodies, the precipitates were separated by SDS PAGE and tested for bound ARF6 by immunoblotting against HA.

In the present study then, we therefore collected urine from prospective study patients and tested ammonium sulfate precipitates of urine for natriuretic activity in a transwell culture system.

The precipitates were completely washed with PBS and tested by immunoblotting.

Immune complexes were precipitated and washed.

The precipitated RNA was resolved in 100 µl of sterile DNA free water and tested on a gel, before it was used for cDNA synthesis and qPCR analysis.

To further establish an interaction between RNAPII and centromeric proteins, we performed the reciprocal experiment, precipitating RNAPIIS2P from solubilized chromatin, and testing for centromeric partners.