MyoD was not detected in any of the E47/E12 precipitates (data not shown).
We found that early hours after injury, Ndel1 co-immunoprecipitates with these truncated fragments but ERK and importins were not found in the co-precipitates (data not shown).
Using the standard conditions of the cell-free expression system, the gp91phox truncated proteins were expressed as precipitates (data not shown).
When MBP-ProSAP2/Shank3 SAM) MBP-ProSAP2/Shank3 SAMequimolar amount of zinc, the majority of the protein precipitates (data not showas.
Eventually, the AuNPs aggregated completely and precipitated (data not shown; the UV/Vis spectra are shown in Figure S10, right).
In contrast, when MBP-Shank1 SAM) MBP-Shank1 SAMubated with zinc, only a minor fraction of the protein precisincubatedata not showith
In addition, we could not find a direct interaction between CED-4 and CED-9 in co-immunoprecipitation experiments and upon mass spectroscopy of CED-4 precipitates (data not shown).
Partial blockage of one of the tubes will also precipitate erroneous data collection.
FilGAPC32 tagged to a maltose binding protein followed by hexahistidine (MBP-His), however, did not pull down with FLAG-FLNa (Figure 1B), and a synthetic peptide of FilGAPC32 immobilized on Sepharose also did not precipitate FLNa (data not shown).
The precipitating particle data are used to validate the optical auroral poleward boundary location along the satellite tracks.
