Sentence examples for precipitate amplification from inspiring English sources

Antibodies are listed in Table S4, and primers for precipitate amplification are listed in Table S5.

67 It also generates a magnesium pyrophosphate precipitate during amplification, which can be used for detection by measuring the turbidity of the solution.

To further evaluate the effect of genomic DNA on downstream reactions, RNA samples processed with each method were amplified and the products of amplification precipitated with LiCl to preserve high molecular weight species.

The following primers were used for amplification of precipitated DNA.

PCR amplification of precipitated chromatin DNA revealed promoter occupancy of these transcription factors.

20 ug of amplification products were precipitated and mixed with 100 ug COT-1 DNA (Roche).

After amplification, plasmids were precipitated, washed and resuspended in sterile TE at 1 mg/ml and 100 µl used for insufflation.

Tailed cDNA was precipitated and used for final amplification with gene-specific primers and 5′ RACE abridged anchor primer provided in the kit.

Therefore, after amplification, we first precipitated the magnesium pyrophosphate to the bottom of the reaction tube by centrifugating the LAMP reaction solution for 10 seconds using a small desk-top centrifuge (6,000 rpm).

The amplification product was precipitated using 70% ethanol in the presence of 20 μg glycogen (Ambion), washed once in 500 μl ice-cold 70% ethanol and re-suspended in 20 μl of nuclease-free water (Qiagen).

Amplification of picogram precipitated DNA was performed by adding carrier up to a total of 500 pg, 1000 pg or 2 ng as indicated using purified, chromosomal E. coli DNA, sonicated to a size distribution of 200 500 bp (Diagenode current protocols).