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Despite these precautions, differences in gene expression were seen between the cases (Fig. 1).
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The use of microarrays to identify candidate reference genes for RT-qPCR normalization has been successful, but this extrapolation requires some precautions due to differences in the choice probe sequences between the two technologies (e.g. Affymetrixprobes typically target the 3' region of a transcript).
The degrees of alert differed for each military service while reports from correspondents of The New York Times showed wide differences in precautions at embassies and among private citizens.
Taking this precaution ensures that any differences in root length between the genotypes compared with the controls are not confounded by mechanical pressures.
The analysis of both raw and percent scores is widely employed in mouse studies [60], [62], [65], [66], [67], [68] as a necessary precaution in case experimental groups show large differences in their startle reactivity.
While these precautions have been taken into account, in our opinion, especially the major differences in complication rate and length of hospital stay cannot solely be explained by the limitations in the design of the included studies.
As a precaution, a parametric test (ANOVA) was also used, without finding any significant differences in cytokine responses between the two groups.
This observation further supports our interpretation of unequal gender relations where differences in which wary sexual behavior among females is a precaution taken in order not to risk sexual exploitation by the dominant gender.
If the level of vitamin D at the time of surgery should influence prognosis, the mechanism must be differences in perioperative resistance to cancer dissemination and the logical precaution would be to ensure a high preoperative vitamin D level.
This contradictory finding may reflect differences in case mix, frequency of patient movements outside the ICU, or compliance with standard precautions and isolation practices [ 39].
This is a precaution to avoid what are known as "batch effects," variations that can crop up because of tiny differences in the way samples are handled.
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