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The assay conditions were controlled, standardized, and pre-optimized to ensure optimal repeatability and reproducibility of the assays.
In Section 4, we apply the gradient-free Nelder-Mead simplex optimization to obtain pre-optimized guess pulses, which then become the starting point for an optimization with Krotov's method.
In contrast, the optimization starting from a pre-optimized pulse has no plateau (note the log-scale of the x-axis); both (J_{T}^{mathrm {sm}}) and (J_{T}^{mathrm {geo}}) converge at roughly the same rate.
Owing to the special features and parallelism added for DSP operations, conventional programming languages such as C do not always produce the most optimal results, and rely on pre-optimized library calls for common algorithms and functions.
Then coupled with the pre-optimized results of the first step, the optimal power split among the engine and two motors could be obtained and then constructed as simple lookup-tables, which have great potential for practical applications.
This pre-optimized pulse then provides a good starting point for further optimization using a gradient-based method.
In the pre-optimized case, the guess pulse was the result of a simplex optimization, see Section 4.2 for details.
Table 2 shows that optimization with (J_{T}^{mathrm {geo}}) requires significantly less propagations (which are directly proportional to CPU time) than optimization with (J_{T}^{mathrm {sm}}), for both direct and pre-optimized strategies.
And so, pre-optimized performance is initially of interest.
Laccase was therefore produced under pre-optimized conditions for purification and immobilization studied.
The correction to the pre-optimized guess pulse is shown in Figure 4.
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