Pre-brewing pre-moistening: Coffee is pre-moistened.
Each disinfectant was sprayed to completely wet the inoculated surface and allowed to remain in contact with the surface until completely dried (~10 minutes) with no surface wiping or allowed to remain in contact with the surface for 3 minutes followed by a manual wipe using sterile gauze that was pre-moistened with the same disinfectant.
The various sampling methods are described below: A total of 180 gauzes were pre-moistened in Bolton broth (without supplement) and the different surfaces were wiped vigorously for 30 s. Gauzes were placed into a jars with 50 ml Bolton broth, without supplement.
"Pull-off" bond results show increased tensile bond strength when the concrete surface is pre-moistened.
The socks were made of jersey material that was factory pre-moistened with 15 mL distilled water.
Cellular skin samples were collected by swabbing human skin or a touched object surface with a sterile water pre-moistened sterile swab.
The sterilized seeds were germinated on sterile filter paper pre-moistened with distilled water at 25°C in the dark for 3 days.
Before use, they were pre-moistened in Bolton selective enrichment Broth (Oxoid CM0983, Hampshire, UK) without supplement, placed in a sterile jar and stored at 4°C.
The slides were sampled with sterile swabs (BD BBL™ CultureSwab™, Becton Dickinson, Cockeysville, MD) pre-moistened with saline, transferred into an anaerobic chamber, spread onto pre-reduced C. difficile selective media and incubated as described previously.
The inoculated surfaces were then sampled with sterile swabs (BD BBL™ CultureSwab™, Becton Dickinson, Cockeysville, MD) pre-moistened with saline, transferred into an anaerobic chamber, spread onto pre-reduced C. difficile selective media and incubated as described previously.
Sterile swabs (BD BBL™ CultureSwab™, Becton Dickinson, Cockeysville, MD) pre-moistened with saline were applied to the surfaces and plated directly onto selective media plates and the number of colonies recovered was counted.
