The representativeness of online data collection is also established to the extent that it follows the procedure used in this survey, i.e. stratified random sampling in a pre-existing large representative panel of the whole population [38], [39].
Survey participants were drawn from two sources: (1) a pre-existing cross-Canada panel of individuals with identified health conditions, maintained by Harris Interactive, a professional polling firm; and (2) patients recruited by the investigators through family physicians' offices and specialty clinics in the vicinity of Hamilton, Ontario, Canada.
A cross-sectional online survey was conducted in five European countries (France, Germany, Italy, Spain and UK) and USA using pre-existing market research panels.
The study participants will be members of a pre-existing, national online survey panel.
Women were recruited using a pre-existing, non-probability web panel from Harris Interactive (HI).
(Top set of panels) Pre-existing resistance means that the 'moderate is best' conclusion is spread out more widely over the parameter space; the DR strain does not benefit as much from DS growth.
Screening through the UL/ b′ region with a panel of pre-existing FIX BAC deletion mutants (Hahn et al., 2004) ruled out UL128, UL129, UL130, UL131a, UL132, UL148a-d, C-orf23, C-orf25, and C-orf26 in regulating the TRAIL DRs (data not shown).
With an achieved sensitivity of 0.02% mutated alleles in the background of wild-type alleles for KIT exon 17 p.N822K, p.N822K and p.Y823D mutations on the MiSeq™ (Illumina) with the AmpliSeq panel, no pre-existing subclones were detected with any of the three assays.
(Bottom set of panels) Without pre-existing resistance, there is more clear separation between the parameter regimes.
[Editors' note: the author responses to the first round of peer review follow.] We have performed additional experiments and analysis; included 2 new figures; added new panels on pre-existing figures; increased the number of experiments on previous figures; and edited the text according to the editorial and reviewers' comments.
