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Inherent site variations offered main determinants of tree heterogeneity, superimposed by human modification to satisfy pre-determined site functions.
Instead of the top seed hosting, the league has switched to a pre-determined site with Portland's Providence Park serving as the host venue – an unfriendly stadium in a fierce Cascadia rivalry.
We previously developed an accumulative gene integration system (AGIS), in which target gene cassettes could be repeatedly integrated into a pre-determined site on a plasmid or cellular genome by recombinase-mediated cassette exchange (RMCE), using Cre and mutated loxPs.
To perfuse a site in the LH, paraventricular (PVN) or ventromedial nucleus (VMN), a concentric 29-23 ga push-pull cannula system was lowered to a pre-determined site, in most cases after catecholamine stores had been pre-labeled with [3H]-NE.
LD muscle was dissected from each animal at a pre-determined site within 15 minutes of euthanasia, snap frozen under liquid nitrogen and stored at -80°C.
The phragmoplast which is made of rod-like proteins called microtubules and actin filaments—starts at the centre of the cell and expands towards a pre-determined site on the existing cell wall.
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It has been reported that nucleation of ferroelectric domains occurs at pre-determined sites in the vicinity of structural defects [1].
All muscles were dissected from the animal within 15 minutes of euthanasia, weighed and samples collected at equivalent pre-determined sites before freezing in liquid nitrogen.
In addition, incorporation of carbohydrates on proteins at pre-determined sites using the genetic machinery is still a rather difficult task.
To overcome this, we used an alternative methodology to express Tau where Tau transgenes are targeted to pre-determined sites in the Drosophila genome to control for any positional effects and permit direct comparisons of toxicity.
Herein, we cover recent clinical developments of carbohydrate-based vaccines and describe how novel cutting-edge methodology for the total synthesis of oligosaccharides and for the precise placement of carbohydrates at pre-determined sites within a protein may be used to further improve the safety and efficacy of glycovaccines.
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