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First, expression of genes at 48 H and 14 D after immobilization was compared to baseline (PRE) using standard paired t-tests to define the early and late transcriptional response to immobilization, respectively.
Serum ferritin was assessed at PRE using an AU5800 immuno-turbidimetric assay (Beckman Coulter, Lane Cove, Australia) to identify iron-deficient participants.
These new restraints were derived from Mn2+-induced paramagnetic relaxation enhancement (PRE) using the ratio of signal intensity (I0/ IMn) determined from 2D spectra collected at 0 μM MnCl2 (I0) and 10 μM MnCl2 (IMn).
We identified transcription factors that are likely to bind the PRE, using competition gel shift and gel supershift: Activator protein 2 (AP2), nm23 nucleoside diphosphate kinase/metastatic inhibitory protein (PuF), and specificity protein 1 (SP1).
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The iPhone, G1, and the Pre use capacitive screens, giving them that hard, smooth, glide-friendly feel.
Pre-amplification: Prior to PCR, cDNA samples were pre-amplified using Taqman PreAmp Master Mix Applied Biosystemss, Foster City, CA).
(B) Data after pre-processing using RMA.
The samples were chemically pre-cleaned using the RCA method.
The survey was then pre-tested using cognitive interviews.
Oxide buffer layer was pre-sputtered using RF sputtering technique.
RS fMRI data were first pre-processed using Statistical Parametric Mapping (SPM5).
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