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EBV-negative CNE-2 cells pre-stained with 1 μM CellTracker Red dye (Invitrogen) were co-cultured with GFP-Akata cells pre-stained with 2.5 μM CellTracker Green dye (Invitrogen) at 1 10 ratio for 12 h.
Some experiments were performed with fibroblasts alone and some by simultaneously seeding fibroblasts and osteoblasts (pre-stained with different fluorescent markers; Molecular Probes) to ascertain competitive cell adhesion.
The SeeBlue Plus2 Pre-Stained Standard was purchased from Invitrogen (Carlsbad, CA. Catalog No. LC5925).
Molecular weight markers See Blue Plus2 Pre-Stained Standard (Invitrogen, #LC5925) was used to determine the size of the antigen.
Hi Alex, I run a company that does Pre-Stained Underwear (underwear that has shit stains printed in them).
The cabins, which vary in size from 10 by 12 feet ($2,375) to 10 by 24 feet ($4,200), are made of pine and arrive pre-stained.
Lane 3: Pre-stained marker.
Lane M, pre-stained Novex-Invitrogen protein marker.
One well was loaded with 4 μl of HiMark™ Pre-Stained Protein Standard (Novex®).
Novex™ Sharp Pre-stained Protein Standard (Invitrogen, Carlsbad, CA) was used for the protein ladder.
Electrophoresis was subsequently performed in 1.0% agarose gel pre-stained with SYBR Safe (Invitrogen, Paisley, UK).
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