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In the present work, we have identified a putative PRE site located in position -1,504 bp relative to the TSS of GATA3.
Since H3K27me3 is catalyzed by the histone methyltransferase EZH2, we tested progestin regulation of EZH2 binding to the putative PRE site located in GATA3 promoter.
We underscored the relevance of GATA3 repression in PR-induced cell proliferation, and described the capability of PR to co-recruit EZH2, the catalytic subunit of PRC2 to the potential PRE site in GATA3 proximal promoter.
By performing chromatin immunoprecipitation (ChIP) assays we found that PR was significantly recruited to the potential PRE site in the presence of MPA both in T47D and in BT474 cells (see Additional file 1: Figure S3).
Taken together, these results demonstrate a PR-mediated GATA3 transcriptional repression through co-recruitment of PR and EZH2 to a putative PRE site at the proximal region of GATA3 promoter, with the concomitant increase of H3K27me3 and decrease of chromatin accessibility, as depicted in Figure 2G.
Similar(54)
For the vg PRE, we present a mutational analysis, identifying an essential function in silencing for a motif (GTGT) that was previously discovered by bioinformatic sequence mining to be enriched at many PRE sites [ 8].
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Update: Palm Pre community site PreCentral last week launched a webOS App Gallery destination.
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