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The PRE sample also exhibited the highest dispersion and activity for the dehydrogenation of 2-butanol to MEK, thus the PRE sample was selected as the best suitable catalyst.
Crt was not detected in one Pre sample.
As negative control, protein A-Sepharose was pre-blocked with 10% bovine serum albumin and then incubated with the lysate (1 mg/ml) without previous incubation with the primary antibody ("pre" sample).
Since some colic horses had spent some time at the clinic by the time the PRE sample was taken, this sample may have been influenced by the volume of administered fluids and the analgesic substances provided to the horse.
In five horses, a sample obtained on arrival (not reported here) indicated that different degrees of hemodilution (decreases in Hb, Hct, serum protein and albumin) had occurred before the PRE sample.
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For "pre" samples, urine was collected just before intra-peritoneal injection.
"Pre" samples were tested at a dilution of 1 100, and "Post" samples were tested at 10 μg/mL.
Half of the pre-sample was asked to first act with the fastest-route goal and then in the more conservative way; vice versa for the other half of the pre-sample.
Heckman's method is based on available pre-sample information with which the conditional distribution of the initial values can be approximated via a reduced form.
To reduce the number of frames used by the clustering algorithm some authors pre-sample the original video, as proposed in [24].
Pre-sampling of the FL and; 3.
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