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(C) The PRE measurement provides long-range structural constraints.
The PRE measurement provides the long-range distance relationship between protein nuclei and paramagnetic center (Liu et al. 2015), and was obtained upon site-specific conjugation of a maleimide-EDTA-Mn2+ probe (Liu et al. 2012) to D334C/C336S double mutant of the ZFD.
This corroborates the PRE measurement at the higher concentration.
The error bar indicates 1 SD in the PRE measurement.
Based on the PRE measurement, the closed-state population decreases by ∼50% upon E64RP mutation.
After randomisation but before pre measurement, five practices withdrew: one in the intervention group and four in the control group (no data available).
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Error bars indicate the SD in PRE measurements.
SIrb was not significantly different between post measurements and pre measurements (p = 0.952).
Paramagnetic relaxation enhancement (PRE) measurements were done on azithromycin in NaDC.
Participants were instructed to abstain from caffeine that morning and exercise for 24 h prior to PRE measurements.
PRE measurements were carried out at 300 μM EIN, 300 μM HPr, and 150 mM NaCl (Fawzi et al., 2010 ).
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