These cells were co-cultured with autologous PBMCs pre-labeled with CFSE.
Notch1 ECD-Fc fusion protein (Notch1 amino acids Ala19-Gln526) (R&D Systems) was pre-labeled with europium (Eu) reagent according to the manufacturer's procedure (PerkinElmer).
Monocytes were pre-labeled with 1 µM H2DCFDA (Molecular Probes, Eugene, Or) for 20 minutes at 37°C using the manufacturer's directions.
Each OIU package was pre-labeled with a unique identifying number.
Very occasionally, NCFR cells were not pre-labeled by RITC-dextran.
The membranes were hybridized with DNA oligonucleotides complementary to the miR394 sequence that was pre-labeled with γ-32P-ATP.
For SILAC quantitation, cells were pre-labeled by propagation in SILAC media and pooled prior to processing together.
We assayed gametocyte emergence by measuring rupture of erythrocytes pre-labeled with a fluorescent α-mTER119 antibody by fluorescence microscopy.
In some experiments, monocytic EPCs and HUVECs were pre-labeled with PKH67 (Sigma, St . Louis MO, USA), and Dil-acLDL, respectively, and cultured together in Matrigel® [ 15].
Study agents were pre-labeled by Shantha Biotechnics personnel, who were not involved in the conduct or monitoring of the trial.
