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For the PLL immobilization, Au surface was pre activated with 11-MUA that provides negative attraction force for the immobilization of positively charged PLL peptide [4].
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Subsequently, the muscle preparations were pre-activated by switching to an activation solution at 1°C.
Rubisco was pre-activated in 100 mmol/L NaHCO3 on ice for 30 min.
In order to decorate with metal nanoparticles, TiO2 nanostructures were pre-activated by the method as described in Ref. [14].
When required, monocytes (2×106 cells/well/1 ml) were pre-activated by MPT (6 µg/ml) in 24-well Ultra Low Attachment plates (Corning).
HUVEC monolayers were pre-activated with 25 ng/ml TNF-α for 6 h to upregulate the expression of adhesion molecules before the tumor migration assay.
Four medical teams (UME) are pre-activated.
Both populations worked better than sorted tTreg cells, likely because they are pre-activated.
The de-activation of pre-activated dopants is observed and a possible mechanism for such a de-activation process is proposed.
This phenomenon might be explained by the fact that Vγ9/Vδ2 cells from active patients are pre-activated in vivo.
These cells are in a pre-activated state and they progressively express activation markers after cultivation [ 18].
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