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gD-TMEFF2-ΔFS I and TMEFF2-ΔFS I/II were PRC amplified with the same reverse primer 5'-CGACTCTAGATTAGATTAACCTCGTGGACGCT-3' and either 5'-CTGCTCGAGTGTGATATTTGCCAGTTTGGTG-3' or 5'-CTGCTCGAGACACCACATACCTTGTCCGGAAC-3' as forward primer, respectively.
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An 422 bp fragment of the prC promoter region without 5'-AGGGG-3' was amplified by PCR with prC genomic DNA as template and using primers PrCF2 (5'- GAA GAT CTC TGT GGG TTC GTC GTA -3'), PrCR2 (5'- GCT CTA GAC GGT TTG TTG CTT GGT GAA G -3') that contained BglII and XbaI restriction sites.
This effect is amplified for larger PRC amplitudes, reflecting the fact that if the phase shift of (varDelta _{mathrm{exc}}) is large, the oscillator will spike before the inhibition arrives.
Briefly, an 844 bp fragment of the prC promoter region containing 5'-AGGGG-3' STRE sites was amplified by PCR with genomic DNA as template and using primers PrCF1 (5'- CGG TAC CGG TCT CTT TTC TAG TTT G-3'), PrCR1 (5'- GGG GAT CCT TTG TTG CTT GGT G -3') that contained KpnI and BamHI restriction sites.
Comedy amplified it.
It amplified the effects.
It almost sounded amplified.
No amplified anything.
"Poor performance gets amplified".
It is themselves, amplified.
Sometimes it's amplified.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com