All isolates were identified by standard clinical practices as previously described [ 18].
The Gene Composer protocol for PCR assembly (PCA) of synthetic genes from designed oligonucleotides (Oligo-sets) is illustrated in Figure 8 and is based on an amalgam of the best practices in previously described PCA methods [ 30- 34].
Primary cells were isolated from surgically resected DD cord and adjacent, phenotypically normal palmar fascia using routine tissue culture practice as previously described [ 34].
Under-recognition of mental disorder in general practice is previously described [ 13, 17, 29], and is completely in line with the general practitioners own descriptions regarding this issue.
Missing or lower relational coordination scores reported with practice nurses may reflect limited interaction with practice nurses as previously described and may also reflect 40% of general practices not employing a practice nurse [ 55].
Adult Long Evans rats were purchased from Charles River Laboratories (Hollister, CA), and husbandry practices were as previously described (Yang et al. 2009).
Details of the chromatography and mass spectrometry conditions, the criteria used for chemical identification and quantification, and the quality assurance and quality control practices have been previously described (Ikonomou et al. 2001).
Penile bloodletting practices shared common underlying cultural meanings, significance and motivators based on constructions of maleness, masculinity and gendered societal roles, analogous to the practices and contexts previously described by in a variety of settings in PNG [ 20, 22, 23, 43].
This suboptimal practice was also previously described in similar settings in other developing countries [ 36, 45].
Sociodemographic criteria, like age or sex, affected the GPs' practices and difficulties, as previously described [ 3, 18].
