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Both intein-based approaches require a number of critical steps and conditions to successfully orient and bind two precursor proteins and the conditions often cannot be practically fulfilled.
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While the 1898 act was designed to meet the need for a high-seas battle fleet, Tirpitz's Second Fleet Act of 1900 laid down an ambitious program to build a larger and more modern oceangoing fleet that the navy was never able to practically fulfill.
Boss professors are busy participating in conferences, developing guanxi, and grabbing projects and funds, while young investigators have to practically fulfill these projects and write papers with the names of the boss professors in the author list, sometimes as the first author, even if the boss professors do not know what was written in the paper.
It needs to be emphasized that the case, when parameters vary over time, while practically significant, do not fulfill the original assumptions of the filter.
With an additional experiment, the scaling law was found to be practically accurate for model prototype pairs of different materials if their similitude requirements were fulfilled.
These efforts to emulate the past, however, have remained subsidiary to the main surviving forms of pageantry, namely the parade, the carnival, and the festival procession, at least one of which is practiced in practically every nation and which fulfill the assertion of national or communal identity in modern mass societies.
More fulfilled?
Fulfilled desire.
Destiny fulfilled.
Ambition fulfilled?
Caroline fulfilled her wish.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com