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The PEO4 and PEO1 cell lines were obtained from F. Couch (Mayo Clinic) in 2013 and viably stored until 2016.
Cells liberated from surfaces with BamHI could be flushed from flow chambers and viably recovered while the majority of cells not bearing enzyme recognition sequences were retained on the surface, and DNA-tethered cells could be nonspecifically recovered viably from surfaces after Benzonase treatment.
PBMC were isolated from freshly drawn whole blood by ficoll-hypaque gradient and viably cryopreserved.
Lymphocytes from mammalian blood can be isolated and viably frozen using a 10% DMSO solution.
Peripheral blood mononuclear cells were isolated by Ficoll from whole blood samples and viably frozen in DMSO.
Cells were mixed with 10% DMSO with 90% fetal calf serum and viably frozen in nitrogen vapor in aliquots of ~106 cells per ml.
Peripheral blood mononuclear cells were isolated by Ficoll from whole blood samples and viably frozen in DMSO prior to flow cytometric analysis of Aiolos expression.
Among other points of agreement, he stressed the need to build resilience in local communities so that they are better prepared when emergencies arise and can recover from disasters more quickly and viably.
Peripheral blood mononuclear cells (PBMCs) were freshly isolated from heparinised blood of patients or healthy donors by Ficoll-Hypaque gradient (Lymphoprep, Fresenius Kabi Norge Halden, Norway) using standard procedures and viably frozen at -180°C until use.
S. aureus strains Newman and ATCC 25923 were grown in TSB medium (18 h, 37 °C), washed twice with a large volume of PBS, and viably labeled with 0.1% FITC in PBS (37 °C, 2 h).
Peripheral blood mononuclear cells (PBMCs) were freshly isolated from heparinised blood of patients by Ficoll-Hypaque gradient (Lymphoprep, Fresenius Kabi Norge Halden, Norway) using standard procedures and viably frozen at -180°C until use.
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