Cassava, a non-grain and high starch content crop, is recognized as an economical and practical substrate for industrial fermentation.
In this study, insoluble α-cellulose is proposed as a more practical substrate to screen for purified CBHs and EGs.
In this study, insoluble α-cellulose was found to be an excellent practical substrate to characterize and screen for purified CBHs and EGs.
This study investigates how these cellulases adsorb and hydrolyze insoluble α-cellulose − considered to be a more practical substrate which mimics the alkaline-pretreated biomass used in biorefineries.
Thus, screening experiments need to be conducted with a more practical substrate such as α-cellulose so that proper cellulases are selected which best hydrolyze the biomass actually used in biorefineries.
As α-cellulose mimics the alkaline-pretreated biomass used in biorefineries, α-cellulose is suggested as an excellent substrate in early experiments to screen for apt cellulases to process practical cellulosic substrates.
In this work, practical SERS substrates based on deposition of silver nanoparticles (Ag NPs) on commercially available low-cost filter paper were prepared by simple silver mirror reaction in a large scale, and utilized for rapid, portable and accurate identification and detection of pesticide residues at various peels.
Since lignocellulose pretreatment in biorefineries significantly alters the structure of cellulose, cellulases should be characterized with other practical cellulosic substrates that represent other pretreatment techniques [ 105, 106] (such as ammonia fibre explosion, ionic liquid process, organosolv process and steam explosion).
The method integrated the advantages of high sensitivity and repeatability, and would significantly facilitate practical SERS substrate preparation.
Considering their low cost, portability, simplicity and high sample collection efficiency, Ag NP-decorated filter paper, as practical SERS substrate, are used in solving critical problems for detection of pesticide residues at various peels.
