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Thus, females were exposed to vehicle, EB, MID DPN, PPT (1 mg/kg) or DPN+PPT (1 mg/kg of each) by sc injection from PNDs 0 to 3. The animals were then sacrificed by rapid decapitation on PND 24 (prior to pubertal onset) or 33 (just after pubertal onset) and the brains rapidly removed and flash frozen on dry ice then stored at -80°C.
For this experiment, only the mid-level dose of DPN was used, a group receiving 1 mg/kg PPT and a group receiving both DPN and PPT (1 mg/kg each) were included to determine if the co-administration of these compounds could replicate the masculinizing effect of EB.
We witnessed three technical errors when healthcare providers performed PPT: (1) air drawn into the intravenous line and inability of the provider to problem solve, (2) stopcock toggling error leading to an attempt to 'pull' from the patient instead of the bag and (3) stopcock toggling error leading to an attempt to 'push' fluid back into the bag of saline rather than into the patient.
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INT 4 PPT -1, senior clinician Equal to their value for building technical competence, many participants saw trial training, knowledge sharing and experience exchange as key enablers for increasing awareness, confidence and motivation.
INT 4 PPT -1, senior clinician The problem is that this [clinical trial research] will take lots of your time and while it is possible to reduce your clinic hours, this means you would lose money.
Although O. latipes can adapt to varying salinity environments to some degree, the adaptive capacity of O. latipes is lower than that of O. melastigma, which can thrive in water of varying salinity ranging from 0 to 35 ppt [ 1 ].
Pelvic floor muscle function The following measurements will be done by the physiotherapist at the second (MT-PPT 1) and final visit (MT-PPT 2).
Shortly before randomisation (M-PPT 1) and at six month, adjacent to M-WB 3, measurements at the physiotherapist will take place (M-PPT 2).
In addition, we attempted to identify members of PPT-1 and/or APT-1 families in Pfam databases (http://pfam.sanger.ac.uk/) [ PF02230 (APT-1) and #PF02089 (PPT-1)] [65].
In addition, we searched for members of APT-1 and/or PPT-1 families in Pfam databases (http://pfam.sanger.ac.uk/) [ PF02230 (APT-1) and #PF02089 (PPT-1)].
First, we performed a Blast search for nonredundant human protein sequences which align to PPT-1 and/or APT-1 protein sequences.
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