Sentence examples for powdered tissue from inspiring English sources

Powdered tissue was placed in 1.0 ml of pre-warmed (65°C) extraction buffer (2.5% w/v CTAB, 1.5 M NaCl, 25 mM EDTA, 100 mM Tris HCl pH 8.0, 1.0% w/v polyvinylpyrrolidone) in a 1.5 ml microcentrifuge tube.

For the breast tumor tissue lysis, the tissue was ground with a mortar and pestle in the presence of liquid nitrogen to create a powdered tissue.

Total RNA was isolated from 30 mg of powdered tissue using Qiagen RNeasy Mini kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.

The tissue was ground in liquid nitrogen and proteins were extracted in 3 ml g−1 powdered tissue of extraction buffer (20 mM Tris-HCl, pH7.5; 300 mM NaCl; 2 mM MgCl2; 5 mM DTT; 2% PVPP; EDTA-free protease inhibitor cocktail (Roche)).

RIPA buffer (50 mM Tris-HCl pH 7.5, 105 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 2 mM EDTA) was added to the powdered tissue, the sample vortexed for 60 seconds, and placed on ice for 45 minutes.

Powdered tissue was placed into an RNase free tube containing 20 ml of Trizol (Invitrogen).

Specific polyphenolic compounds were extracted by grinding 0.1 g frozen, powdered fruit tissue in 1.5 mL pure methanol.

The powdered bone tissue was transferred to a microtube for addition of the TriReagent (1 ml) and extraction and isolation of the RNA.

Briefly, dried and grounded into powder tissue samples were heated for 1 hour at 65°C with water and further filtrated through GF/A filters which was followed by washing several times with different organic solvents (in turn: ethanol, acetone, diethyl ether).

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Total RNA was extracted from frozen, homogenized and powdered tomato fruit tissue using the CTAB (hexadecyltrimethylammonium bromide) method [ 48].