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On the following day, 0.2 mL of 0.1% croton oil in Freund's Complete Adjuvant (FA; v/v) was injected into the air pouch cavity.
In another series of experiments, we investigated whether administration of specific anti-inflammatory drugs promotes further blockade of PMN influx to the air pouch cavity of infected mice.
Yet, whether or not LTB4) or PAF) was injected locally into the air pouch cavity to induce an acute secondary inflammatory response, PMN were still efficiently recruited to the infected lungs and 1(d)).
In contrast, a single administration of CP-105,696 did not further decrease LTB4-elicited PMN influx to the air pouch cavity 48 h after inoculating Hc in C57Bl/6 mice).
As shown in Figures 1(a) and 1(b), administration of soluble agonist, such as LTB4 or PAF into the air pouch cavity, elicited an intense inflammatory cell infiltrate consisting primarily of PMN into the air pouch of noninfected mice when compared to vehicle inoculation.
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typhimurium in 1 ml PBS were injected into the air pouch cavities.
Subsequently, murine models of peritoneal [ 7, 11] and subcutaneous air-pouch cavity of MSU-crystals have been utilized to study the inflammatory response [ 7, 18].
Back in the kitchen, Alex Pilas, the restaurant's banquet chef, placed a plastic pouch of broth into the cavity of the presentation pig.
Santos-de-Oliveira [9] showed that ArtinM induces migration of neutrophils to the peritoneal cavity or air pouch of rats and also showed that ArtinM has chemotactic activity for human neutrophils in vitro.
In murine models of crystal-induced inflammation, the injection of MSU into a preformed air pouch or within the peritoneal cavity elicited an influx of neutrophils that was accompanied by the local accumulation of chemoattractants and cytokines.
For swab samples, dacron-tipped applicators were inserted into the oropharyngeal cavity (behind the pouch) or into the cloaca and then submerged and swirled in vials containing 1 mL BA-1 medium and discarded.
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