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Several studies in different animal species have shown positive induction of tumors in both target (cheek-pouch, esophagus and stomach) and non-target (lung and liver) tissues when arecoline (ARC) or areca-nut extract was administered by different means such as oral intubation [ 2], mixed with the diet [ 3], and cheek-pouch application [ 4].
FCFM was performed on precancerous lesions in the hamster cheek pouch model, following topical application of the fluorophores.
Following HP application, the pouches were opened with autoclaved instruments and the samples were returned to individual agarose coated wells.
In application, lithium-ion pouch-format cells undergo expansion during cycling.
The contralateral cheek pouch of the hamster received mineral oil application only.
The dehydrated eggs are packed in containers ranging from small pouches to large drums, depending on their commercial application.
We conduct a techno-economic analysis of Li-ion NMC-G prismatic pouch battery and pack designs for electric vehicle applications.
Topical application of DMBA for 14 weeks induced buccal pouch tumours that showed aberrant expression of cytokeratins, a marker for epithelial carcinomas.
The right buccal pouch of the hamsters was treated by thrice-weekly topical application of 0.5% 9, 10-dimethyl-1, 2-benzanthracene (DMBA) for 6-7 weeks to induce dysplasia (n = 4).
In 32 non-operated control animals, the average tumour yield after 12 weeks' DMBA application was 2·13 carcinomata and 1·22 atypical papillomata per treated pouch.
Figure 2 I shows that the healthy cheek pouch cells collected by swabbing displayed DNA alterations at baseline, prior to fluorophore application and before illumination, which were linked to epithelial keratinization.
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