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Per replicate experiment usually 10 wasps were tested sequentially using the same set of two potted seedlings.
Potted seedlings were grown in a complete factorial design.
These potted seedlings were then transferred to fog cabinets placed in a growth chamber and exposed to simulated neutral (pH 5.6: SiNF) or acid (pH 3.0: SiAF) fog for 6 h (9 00-15:00) each day for 7 days (Ito et al. [2011]).
Potted seedlings were watered every second day.
The juvenile potted seedlings were then transplanted and grown under field conditions.
Individually potted seedlings were inoculated with four apterous adults of A. idaei biotype 1, 14 weeks after germination, and scored for colonisation (presence of both adults and nymphs) 10 days later.
Similar(52)
Chromosome spreads were prepared from root tip protoplasts of young potted P. taeda seedlings (progeny of genotypes LSG-62, B-5-3 and B-145-L) as described [70], [71].
They were released individually from a glass tube, positioned halfway the distance between the two potted cabbage seedlings.
Since then this initial population was reared on potted radish seedlings (Raphanus sativus L). at 25 ± 1°C, 65±5 5% RH and L D = 16:8 h in a separate greenhouse without exposure to insecticides over the past ten years.
Its wall bristles with potted spiky plants instead.
The dish was placed on soil near the foot of a potted cabbage seedling.
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